VASCULAR BIOLOGY Brief report PV-1 is recognized by the PAL-E antibody and forms complexes with NRP-1

نویسندگان

  • Johannes Keuschnigg
  • Denis Tvorogov
  • Kati Elima
  • Marko Salmi
  • Kari Alitalo
  • Tiina Salminen
  • Sirpa Jalkanen
چکیده

1MediCity Research Laboratory, and 2Turku Graduate School of Biomedical Sciences, University of Turku, Turku, Finland; 3Molecular/Cancer Biology Laboratory and Department of Pathology, Haartman Institute and Helsinki University Hospital, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland; 4Department of Medical Biochemistry and Genetics, University of Turku, Turku, Finland; 5National Institute of Public Health and Welfare and Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland; and 6Department of Biochemistry and Pharmacy, Åbo Akademi University, Turku, Finland

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PV-1 is recognized by the PAL-E antibody and forms complexes with NRP-1.

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The prototype endothelial marker PAL-E is a leukocyte trafficking molecule.

Pathologische Anatomie Leiden-endothelium antibody has been used for more than 20 years as a marker for vascular endothelium. Despite its widespread use, the target of this antibody was only recently identified as plasmalemma vesicle-associated protein-1 (PV-1). However, no function has been identified for this molecule. Here we report that activation of human umbilical vein endothelial cells w...

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Molecular identification of PAL-E, a widely used endothelial-cell marker.

The pathologische anatomie Leiden-endothelium (PAL-E) antibody has been used for almost 20 years as a specific marker for vascular endothelial cells. Due to the fact that this antibody works only in very limited applications, the molecular identity of PAL-E has remained unknown. In this work, we demonstrate by double stainings, cross-immunoprecipitations, and transfectants that the PAL-E antige...

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تاریخ انتشار 2012